Genomic comparison of Clostridium chauvoei isolates from classical and visceral clinical manifestation

Complexity of toxins from Clostridium septicum and Clostridium chauvoei.

Production of toxins by Clostridium septicum and Clostridium chauvoei has been the subject of many conflicting reports. Roux and Chamberland (1887) first noted that 3 to 4 day filtrates were antigenic; conferring on guinea pigs an immunity against infection with viable organisms. Production of a lethal toxin by C. septicum was however, demonstrated by Leclainche (1898) and Leclainche and Morel ...

Sequencing and comparative analysis of flagellin genes fliA and fliB in bovine Clostridium chauvoei isolates

Background: Clostridium chauvoei is the etiological agent of blackleg as an endogenous infection in cattle.  Flagella have been known to play a critical role in the protective immunity of animals to clostridial infections. C.chauvoei has two copies of fliC gene, namely fliA and fliB. OBJECTIVES: The aim of this study was the determination and nucleotide sequence analysis of both copies of fliC ...

Detection and identification by PCR of Clostridium chauvoei in clinical isolates, bovine faeces and substrates from biogas plant

BACKGROUND Clostridium chauvoei causes blackleg, an acute disease associated with high mortality in ruminants. The apparent primary port of entry is oral, during grazing on pasture contaminated by spores. Cases of blackleg can occur year after year on contaminated pastures. A method to determine the prevalence of C. chauvoei spores on pasture would be useful.The standard method for C. chauvoei ...

Polymorphism of Flagellin gene (fliC) of Clostridium chauvoei isolates from Iran

Flagellums have been reported to play a critical role in the protective immunity in host. In clostridia genera it is coded by flagellin (fliC) gene. Previous southern blot analysis has suggested that Flic gene may has two copies in genome of C. chauvoei. We studied sequences of two copies (fliA and fliB) of flagellin gene in Iranian isolates. In this study, four specific primers for fliA, fliB ...

Isolation and Characterization of Clostridium chauvoei Strains Isolated from Cases of Blackleg in Cattle in Iran